Guideline for screening antioxidant against lipid‐peroxidation by spectrophotometer
نویسندگان
چکیده
Lipid peroxidation (LPO), an oxidative chain reaction often occurring in the human body and food, is harmful to health. Due its convenience low cost, this guideline highlights benefits drawbacks of LPO models by spectrophotometer vitro vivo. The preparation process mechanism lipid substrates, oxidation triggers detection methods are discussed paper better screen antioxidants. substrates mostly consist rat liver/brain homogenate, microsomal, low-density lipoprotein (LDL), linoleic acid (LA), appropriate for modeling damage a variety situations. Transition metals, ascorbic acid, 2,2′-azobis (2-amidinopropane) hydrochloride (AAPH), sodium Nitroprusside (SNP), 3-morpholinosydnonimine (SIN-1) nicotinamide adenine dinucleotide phosphate (NADPH) make up majority oxidizing triggers. following quantification techniques have been reviewed: (a) thiobarbituric reactive substances (TBARS) assay, (b) measurement conjugated-diene (CV), (c) β-carrot bleaching (d) thiocyanate method, (e) ferrous oxidation-xylenol orange (FOX-2) assay. It can be concluded that more than one test system should applied obtain comprehensive results. In word, provides scientific basis future research on antioxidants screening food systems.
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ژورنال
عنوان ژورنال: eFood
سال: 2023
ISSN: ['2666-3066']
DOI: https://doi.org/10.1002/efd2.80